ASSOCIATION OF HUMAN ABO AND RH (D) BLOOD GROUP SYSTEMS WITH SOME INTESTINAL PARASITIC INFECTIONS IN BABILE DISTRICT, EAST HARARGHE ZONE, OROMIA REGIONAL STATE, ETHIOPIA

Abstract

The ABO and Rh blood groups are the most important blood groups despite the long list of several other blood groups discovered so far. The ABO and Rh blood group frequencies vary worldwide and are not found in equal frequencies even among ethnic groups. In this study, a cross-sectional survey was used to assess the phenotypic, allelic and genotypic frequency distribution of ABO and Rh blood groups and possible associations with intestinal parasitic infections in Babile district, East Hararghe, Eastern Ethiopia A total of 420 study subjects were participated in this study. A total of 420 blood samples and 420 stool samples were collected from study individuals from November 2016 to January 2017 G.C. Stool examination was done by direct wet mount for detection of intestinal parasites. Blood samples were collected from each participant by open slide method and RPR card method. Data were analyzed by using SPSS version 20. Chi-square test (χ2 ) was used to test whether the population is at Hardy Weinberg genetic equilibrium or not, and to assess the association between ABO blood groups and intestinal parasitic infections. Observed difference were considered to be significant at P<0.05.with 95% confidence interval (CI). Blood type O and Rh-positive had the highest frequency while blood type AB and Rh-negative had the lowest frequency in the study participants. The overall phenotypic frequencies of blood type O, A, B and AB were 48.6%, 28.6% and 17.4% and 5.5%, respectively. The overall allele frequencies were 0.693 IO, 0.186 I A and 0.121I B and genotypic frequencies were I OI (0.4802), I A I A (0.0346), I A I O(0.2578), IA I B ( 0.0450), IB I B (0.0146) and IB I O (0.1677) in the study participants. The frequency of Rh+ and Rh- were found to be 98.8% and 1.2%, respectively, in the overall sampl.e The allelic frequencies of ID and Id were 0.891 and 0.109, respectively and genotypic frequencies of Rh(D) blood group were I DI D (07938), I DI d (0.1942) and Id I d (0.0118) in the study participants. The distribution of overall observed frequencies of ABO blood group phenotypes do not differ from those expected under Hardy Weinberg equilibrium, which indicate that the population is at Hardy Weinberg genetic equilibrium. According to this study the prevalence of G. lamblia infection was 103 (24.5%) followed by 103 (24.5%) of E. histolytica 23(5.5%) of S. mansoni. 12(2.9%) of H. nana and 4(0.9%) multiple intestinal parasitic infection. This study shows that phenotypic, genotypic and allelic frequency of ABO and Rh(D) blood group system in the study participants and prevalence of intestinal parasitic infections do not show significant association in the study participants.