INVITRO REGENERATION OF Echinops kebericho FROM SHOOT EXPLANTS

Abstract

Echinops kebericho is an erect massive rootstock bearing perennial herbs or shrub belonging to the family Asteraceae. Echinops kebericho, endemic to Ethiopia is a critically endangered medicinal plant. It is among few important medicinal plants of the country valued primarily for root parts. The seeds of Echinops kebericho characterized by short viability period and lose their capacity to germinate very quickly. This study aimed to develop in vitro regeneration of Echinops kebericho from shoot explants. The study started with seeds germinations on culture jar for two different months. Seeds were sterilized 70% (v/v) alcohol 9 minute and 5.25% (w/v) sodium hypochlorite with added 1-2 drops of Tween 20, rinsed double distilled water and cultured Murashige and Skoog (MS) medium containing 30 g/L sucrose and 8 g/L agar. The shoot induction was MS medium supplemented with different concentrations of BAP and KN with combined 0.5 mg/l NAA. For regeneration and multiplication shoots were different concentration of BAP and KN .The, multiplied shoots were rooted on MS medium supplemented with concentration of IBA and IAA. The highest number of shoots induction was recorded 1.0 mg/l KN with combined 0.5 mg/l NAA (10.30±0.30) of shoot per explants and the shoot length (5.20 cm) were observed. The 1.0 mg/l KN showed maximum shoot regeneration on shoot regeneration media and shoot multiplication media respectively. The highest percentage of rooted plants were obtained from in 1/3 MS basal medium in while maximum root length were recorded on the medium supplemented with 0.5 mg/l IAA were recorded. Among 30 plantlets were hardened and successful acclimatized to the green houses with 80% survival. Overall results of the present study the effect on hormone and techniques of invitro regeneration. In order to see further achievement in vitro regeneration of Echinops kebericho use combination hormone cytokinin and auxin.