ANTIMICROBIAL AND ANTIOXIDANT POTENTIALS OF ENDOPHYTIC FUNGI FROM CORIANDER (Coriandrum sativum L.) LEAF AND SEED

Abstract

Endophytes demonstrate great potential sources for new bio active compounds. Therefore, the present study was undertaken to assess biological activities including antioxidant and antimicrobial potentials of endophytic fungal extracts from coriander leaves and seeds. The samples were surface sterilized before used as endophytic fungal culture. The result of macroscopic and microscopic characterization of the fungal isolates demonstrated the presence of three genera of endophytic fungi including Acremonium sp, Scopulariopsis sp and Peniophora sp. The fungal inoculates obtained from pure culture fermented using rotary shaker.10g of mycelia collected and filtered. Quantification of phenolic content was carried out and the antioxidant activity was conducted based on DPPH radical scavenging activity and hydrogen peroxide scavenging activity. The antimicrobial activity test was conducted based on disc diffusion method and broth dilution methods. Significantly the highest Quantification of phenolic antioxidant content (15.53 mg GAE/10 g DW), DPPH (53.05%) were recorded for Scopulariopsis sp extract from coriander leaf isolate. The highest concentration of the crude extracts (150 mg/ml) presented the strongest antibacterial activity with maximum zone of inhibition (25.77 mm) for Scopulariopsis sp extract against Staphylococcus aureus indicating that Staphylococcus aureus is more susceptible to the crude endophytic fungal extracts. The maximum anti fungal activity with zone of inhibition (31.83 mm) was recorded for Acremonium sp extract of leaf isolate against Aspergillus niger. The endophytic fungal extract presented the strongest antibacterial activity with Minimum Iinhibitory Concentration (3.13 mg/ml) and corresponding minimum bactericidal concentration (3.13 mg/ml) for Scopulariopsis sp against Staphylococcus aureus. Whilst, the weakest antibacterial activity withminimum inhibitory concentration (125 mg/ml) and corresponding minimum bactericidal concentration (200 mg/ml) were recorded for Acremonium sp extract from seed isolate against E. coli. The strongest antifungal activity with minimum inhibitory concentration (1.17 mg/ml) and corresponding minimum fungicidal concentration (1.17 mg/ml) was recorded for Acremonium and Peniophora sp extract from coriander leaf isolate against A. niger, and Acremonium sp extract from leaf isolate against A. niger. It can be concluded from the present result that Scopulariopsis sp isolate extract was found to have the best antioxidant and antibacterial potentials while the Acremonium and Peniophora spp isolate from coriander were found to have the best for anti fungal activities and it can be recommended for microbial production of bio active compounds if confirmed through further investigation.