Abstract:
Milk is an intricate or a complex biological fluid and by its nature, a good grawthmedium for
many microorganisms.Becouse of its physico-chemical properties, its needs strict hygienic
condition to avoid contamination of milk with microorganisms.Therefore, the microbial
content of milk is a major feature in determining its quality.Microbial contamination can
generally occure from three sources such as, with in the udder, exterior of the udder and
surface of milk handling and storage equipment. Thus, this study was carried out to assess the
microbiological quality of raw caw milk and yoghurt sample collected from household and
vendors in Haramaya woreda.A total of 27 milk samples was collected from household and
venders and transported to the laboratoryat <4℃ for total plate count, Coliformcounts,
Enterobacteriaceaecounts, and yeastand mold counts determination. Mean total plate
count,Coliform count, Entrobacteriaceae count,Yeast and mold count for raw milk samples
collected from the households were 5.97±0.88,4.96±0.70, 4.54±0.72,and 3.9±0.64 and
4.1±0.66log10 cfu/ml, respectively. The mean values for raw milk samples collected from the
equipment of the vendors were 6.5±0.77, 5.0±0.81, 4.2±0.60, and 3.5±0.4 and 3.6±0.43log10
cfu/ml for total plate count,Coliform count,Entrobacteriaceae count,Yeast and mold
countsrespectively.The mean total plate count,Coliform count,Entrobacteriaceae count,Yeast
and mold count for yoghurt sample collected from the equipment of the household were
5.74±0.51, 4.9±0.7, 4.54±0.85, 4.4±0.71 and 4.5±0.83log10 cfu/ml respectively.The microbial
counts for samples collected from the equipment of the vendors were 6.2±1.34, 4.2±0.63,
4.2±0.77±, 4.35±0.07 and 4.2±0.14log10 cfu/ml for total plate count,Coliform count,
Entrobacteriaceae count,Yeast and mold count, respectively.To protect the raw and fermented
cow milk from spoilage loss and consumers from milk born public health risk, the availability
of documented information on the microbiological quality of raw and fermented caw milk
across the milk supply chain is critically important as such information may be important for
different organization to undertake relevant development intervention on hygienic practices
essential for safe milk production and handling.Generally, the present study revealed that
milk samples contained higher microbial load than different standards and considered as
substandard which will result in public health hazard to the consumer.Therefore, intensive
study on microbial status of milk in the study sites should be conducted to assure safety and
quality policies to be set to assure the supply of quality milk in the area.It could be concluded
that raw and yoghurt milk samples collected from household and vendors were severely
contaminated with total bacterial count, coliform count,entrobacterial count, yeast and
molds, with exceeding the respective acceptable limits.