Abstract:
Endophytic fungi represent a wide diversity of microbial adaptations that have evolved in
special and unusual environments, making them a great source of study and research for new
drugs for medical, industrial, and agriculture uses.The present study was undertaken to
investigate antioxidant and antimicrobial potential of endophytic fungalextracts ofkoseret leaf
and seeds.The leaf and seed samples collected from the home gardens were used for endophyte
fungal extraction.Endophytes were isolated using Potato Dextrose Agar and the growth tip
examined by macro and microscope following the standard procedures. The antimicrobial
experiment was arranged in a completely randomized factorial design including four endophytic
fungal extracts from leaf and seed samples of koseret at three concentration levels, a solvent
(methanol), and four test pathogens in three replications. Antioxidant activities of the extract
was assessed by 2, 2- diphenyl-1-picrylhydrazyl (DPPH) and Hydrogen peroxide
whereas;antimicrobial activities were used disc diffusion and broth dilution methods. The
significantlyhighest phenolic content (13. 48 gm GAE/g) and radical scavenging activity
(54.74%), tested with DPPH displayed by Alternaria sp, whilst Aspergillus sp extract has been
recorded as the least phenolic content (7.67mg GAE/g) as well as least radical scavenging
activity (22.43%) with DPPH. The antibacterial activity in concentration(125mg/ml) of the
crude extracts has been recorded the diameter zone of inhibition ranging from (14.47 to 21.50
mm)against both tested strains E.coli and S. aureusrespectively. In the same concentration
(125mg/ml) of the extract has been presented the diameter zone of inhibition ranging
from(16.07 to 25.60 mm) against tested fungal strains. The crude extracts endophytic fungal has
been presented the strongest antibacterial activity with MIC (5.21mg/ml), and MBC
(10.42mg/ml least value) against S. aureus recorded byAspergillus sp and strongest antifungal
activity with MIC(2.21mg/ml), and MFC(10.42mg/ml)least value against both A. niger and A.
versiclorshowed by Alternaria sp.The finding of the present study demonstratedthat the
endophytes extract has indicated higher biological activitiesincluding antioxidant and
antimicrobial potentials
