dc.contributor.author |
gudeta, Nura |
|
dc.contributor.author |
balakrishnan, Senthilkumar Co Advisor (PhD) |
|
dc.contributor.author |
teklemariam, Zelalem Co Advisor Mr. |
|
dc.date.accessioned |
2018-01-28T16:57:55Z |
|
dc.date.available |
2018-01-28T16:57:55Z |
|
dc.date.issued |
2017-11 |
|
dc.identifier.uri |
http://localhost:8080/xmlui/handle/123456789/3298 |
|
dc.description |
87 |
en_US |
dc.description.abstract |
Milk contains abundant water and nutrients with nearly neutral pH which makes it good
media for the growth of microorganisms. Consuming quality deteriorated milk is
hazardous to health. The aim of this study was to determine bacteriological quality,
associated factors and antibiotic susceptibility pattern of targeted isolates of raw cow’s
milk from Adama town cafeterias, Oromia Region, Ethiopia from March 23-June
06/2017. A cross-sectional study on 115 raw cow’s milk samples collected from
randomly selected cafeterias was conducted. Questionnaires were employed to survey
handling practices. About 10-15 mL milk samples collected and transported in an ice
box below 5°C to Oromia Regional laboratory in Adama town for bacteriological
analysis. Serially diluted sample inoculated in duplicate on to Nutrient Agar and Eoisin
Methylene Blue Agar for total and coliform bacteria counts respectively. Colony with
metallic sheen on Eoisin Methylene Blue Agar was presumptively assumed as
Escherichia coli and streaked onto same media to get pure isolates after which were
confirmed by biochemical tests. Mannitol Salt Agar and Salmonella–Shigella Agar
selective media were also used for the cultivation of Staphylococcus aureus and
Salmonella spp respectively. Data were entered into EPI data version 7 and exported to
SPSS version 22 computer soft ware and interpreted using descriptive statistics. Also
Pearson chi square test used to see the association of the potential factors with bacterial
milk contamination. The mean± standard error of total bacterial and coliform counts
was 6.600±0.144 and 4.957±0.104Log10 CFU/mL respectively. About 7.8% (n=6) E.
coli and 23.5% (n=27) S. aureus were isolated but none sample were positive for
Salmonella spp. Antibiotic sensitivity test was done for all E. coli and Staphylococcus
aureus isolates by the Kirby-Bauer’s disk diffusion technique as modified by the Clinical
and Laboratory Standard Institute. All isolates of E. coli were highly resistant to
ampicillin but sensitive to amoxicillin-clavulanic acid, cotrimoxazole, gentamicin,
tetracycline, ciprofloxacin, chloramphenicol, cefoxitin, and ceftriaxone. Most of the S.
aureus isolates were resistant to one or more antibiotics tested. Most resistance was
observed for ampicillin and tetracycline, while 100% sensitivity was recorded for
ciprofloxacin. About 25.9% were resistant to three or more antibiotics (multidrug
xvii
resistant). About 71.3 % samples of raw whole milk collected and analyzed for their
bacteriological quality were substandard in terms of their total bacterial counts. Of the
total samples analyze about 67% were positive for coliforms and all had total coliform
counts above tolerable limit. The poor bacteriological quality is perhaps attributable to
lack of good producing practices during milking and mishandling practices during post
milking. Therefore, adequate sanitary measures and strict monitoring and quality control
measures should be in place at all levels from production to consumption to assure
delivery of safe and quality of raw whole milk to various stakeholders |
en_US |
dc.description.sponsorship |
Haramaya university |
en_US |
dc.language.iso |
en_US |
en_US |
dc.publisher |
Haramaya university |
en_US |
dc.subject |
Raw Milk, Bacteriological Quality, SPC, TVBC, Coliforms, CFU, E. coli, S. aureus. |
en_US |
dc.title |
Bacteriological Quality, Associated Factors and Antibiotic Susceptibility Pattern of the Isolates of Raw Cow’s Milk Collected from Cafeterias in Adama Town, Oromia Region, Ethiopia |
en_US |
dc.type |
Thesis |
en_US |