ISOLATION ANDA IDENTIFICATION OF COMMON PATHOGENIC BACTERIA FROM GUT OF HONEY BEE IN KURFA CHELE DISTRICT, EAST HARARGHE ZONE, OROMIA REGION,ETHIOPIA

Abstract

The study was conducted with the objectives of isolating and identifying of common pathogenic bacterial species fromdigestive tracts of honey bees (Apis mellifera) and determine their antibiotic susceptibility patterns in Kurfa Chale district. A cross sectional type study wasemployed to achieve the objectives. A total 40 pooled samples of live adult honey bee were collected. Summary of descriptive statistics such as frequency and percentages were used to describe the occurrence of bacterial pathogens. The proportion of different bacteria found in the gut were analyzed for the association with study variables by Pearson Chi-square test. P-value less than 0.05 was considered as significant. Out of 40 samples investigated 30 were positive and 10 were negative.From 30 positive sample 28 were targeted bacterial species and the remaining 2 were gram positive bacteria. The overall occurrence of K. pneumonia, E.coli and Serratia marcescenin bee samples were 8 (20%), 7 (17.5%) and 13 (32.5%), respectively. The occurrence of isolated bacterial species was various in different study area.The frequency according to kebele were 9 ( 69.2%),9 (69.2%),10(71.4%) for Arele, Jiru belina and Hula jeneta respectively. Regarding to occurrence in hive types, the frequency of bacterial species identified in studied types of hives was 19 ( 73.1%) and 11 ( 78.6%) for traditional and modern hives respectively. Risk factors such as study area and hive types did not show statistically significant variation on the occurrence isolated bacterial species ( p> 0.05).The bacterial isolates were tested against six commonly use antibiotics using disc diffusion method. Drugs tested were Amoxacilin, ampicillin, tetracyclin,vancomicin, gentamicin and kanamacin. The corresponding results for susceptibility pattern tests carried out indicate that all isolated bacterial species were shown full resistant to ampicillin , amoxicillin ,tetracyclin and vancomicin and in contrast, they indicated greater susceptibility to gentamicin and kanamycin.The present finding used gram stain, morphology and biochemical test to isolate and identify pathogenic bacterial species. Further investigation and experiment will be needed for exact decision regarding isolation and identification of pathogenic bacteria from digestive tracts of honey bee.