SEROLOGICAL DETECTION AND MOLECULAR CHARACTERIZATION OF BOVINE VIRAL DIARRHEA VIRUS (BVDV) FROM SELECTED DAIRY FARMS IN JIMMA TOWN, SOUTHWEST ETHIOPIA

Show simple item record

dc.contributor.author Meseret Mohammed
dc.date.accessioned 2024-12-16T08:32:15Z
dc.date.available 2024-12-16T08:32:15Z
dc.date.issued 2024-04
dc.identifier.uri http://ir.haramaya.edu.et//hru/handle/123456789/7978
dc.description 83p. en_US
dc.description.abstract Bovine viral diarrhea (BVD) is an economically important disease in most cattle-producing countries all over the world including Ethiopia and is caused by Bovine viral diarrhea virus (BVDV). The main purpose of the current study was to identify the type of circulating Bovine viral diarrhea virus in dairy cattle, to estimate the seroprevalence, and assess the risk factors associated with bovine viral diarrhea seroprevalence in dairy cattle in Jimma town of Oromia Regional State, south-western Ethiopia. A cross-sectional study was employed to collect samples and relevant data from November 2023 to April 2024 from 48 dairy farms and 383 non- vaccinated animals. A total of 383 serum samples were subjected for the detection of Bovine viral diarrhea virus (BVDV) antibodies and antigens using a competitive enzyme-linked immunosorbent assay (ELISA) kit (ID Screen® BVD p80 Antibody). In addition, a one-step reverse transcription polymerase chain reaction (RT-PCR) enzyme mix (Qiagen®, California, USA) was used to detect the viral genome in pooled swab samples. Analytical statistics such as chi-square and multivariable logistic regression were used to present study findings using the SPSS version 26 statistical analysis tool. In the present study, a total of 72 (18.8%) (95% CI: 15.0-23.1) animals and 20 (41.1%) (95% CI: 27.6-56.7) farms were seropositive to BVDV antibodies. Based on chi-square analyses, age, history of respiratory problems, breeding system, production system, and animal housing system showed significant association (p<0.05) with bovine viral diarrhea seroprevalence. However, only the production system showed a significant association (p<0.5) at the farm level. On multiple logistic regression analysis, the odds of seroprevalence in adult animals was 2.2 (OR=2.2; p=0.02). In addition, animals with a history of respiratory problems and those housed in head-to-tail arrangements had 2.7 (p=0.205) and 4.8 (p=0.021), respectively the odds of becoming seropositive than their counter categories. All samples tested using RT-PCR and antigen detection ELISA were negative for BVDV antigen. The present study concludes that a considerable proportion of dairy cattle in the study area are exposed to BVDV. However, there is no evidence of persistent infection (PI) among the dairy farms in the study area. Thus, there is a need to apply effective management strategies against BVDV including, vaccination, biosecurity measures, and housing management en_US
dc.description.sponsorship Haramaya University en_US
dc.language.iso en en_US
dc.publisher Haramaya University en_US
dc.subject Bovine viral diarrhea virus, ELISA, Persistent infection, Risk factors, RT-PCR, Jimma town, Ethiopia en_US
dc.title SEROLOGICAL DETECTION AND MOLECULAR CHARACTERIZATION OF BOVINE VIRAL DIARRHEA VIRUS (BVDV) FROM SELECTED DAIRY FARMS IN JIMMA TOWN, SOUTHWEST ETHIOPIA en_US
dc.type Thesis en_US


Files in this item

This item appears in the following Collection(s)

Show simple item record

Search HU-IR System


Advanced Search

Browse

My Account