IDENTIFICATION AND ANTIMICROBIAL SUSCEPTIBILITY PROFILES OF Salmonella SEROTYPE O: 4 (B) GROUP WITH PUBLIC HEALTH AWARENESS AT HARAMAYA UNIVERSITY ABATTOIR, EASTERN ETHIOPIA

Abstract

Salmonella has been found to be the major cause of foodborne diseases and a serious public health problem with an increasing spread of antimicrobial resistant strains. To address this significant issues crossectional study was conducted from November 2016 to February 2017 to estimate the prevalence and antimicrobial susceptibility profile of Salmonella Serotype O: 4 (B) group in beef carcass and environmental swab sample in abattoir at Haramaya University. A total of 484 samples were collected and transported to laboratory and analyzed following methods to detect and identify the Salmonella Serotype O: 4 (B) group. The result revealed that out of 384beef carcass and 100 environmental swab samples examined the prevalence of Salmonella serotype O: 4 (B) group was 25.52 %, 28.0%, 20.0%, 16.0%, and 48.0% of beef carcass, cutting board, hand swab sample, knife and floor sample respectively with overall Salmonella prevalence of 26.03%. Furthermore, Antimicrobial susceptible isolates of Salmonella serotype O: 4 (B) group were showed Ampicilin (AMP- 10 μg) 97.62%, Erythromycin (E-15 μg) 96.03%, Amoxicillin-clavulanic acid (AML-30 μg) 77.78%, Tetracycline (TE-30 μg) 75.39% with the highest resistance and lowest for Nitofurantoin (F-300 μg) 54.76%, Gentamicin (CN-10μg) 45.23%, Streptomycine (S-10 μg) 45.23% and Chloranphenicol (C-30 μg) 16.67% were noticed. Abattoir observation indicates that carcass contamination was occurred due to water splash from floor and consumer may predispose to salmonellosis without considering food hygiene during preparation. Based on result, creating the awareness to reduce the prevalence of Salmonella serotype O: 4 (B) group from beef carcass with antimicrobial resistance is recommended and also detail further study for Salmonella serotypes from serotype O: 4 (B) group and their public Health importance is necessary.