Abstract:
A cross sectional study was conducted from January 2021 to June 2022 in Babile District, East
Hararge Zone, Oromia, Ethiopia, with aim of S. aureus isolation from raw camel milk, to assess
antimicrobial resistance and public health importance.The study was conducted on a total of 223
milk samples from producers, collectors and retailers. For this study both laboratory and
questionnaire based data were collected and analysis by chi-square test(x2
). The overall prevalence
of S. aureus was found to be 18.4 % (41/223).The frequency of isolation of S. aureus were varied
between sources of sample and ranged from 11.7-34.6 %. The prevalence of S. aureus was 11.7 %
(16/137), 20.6 %( 7/34) and 34.6% (18/52) from udders at milk producers, milk from container at
collectors and retailers, in the increasing order. There was statistically a significant difference
(P=0.001) in isolation of S. aureus from milk udders at producer, milk from containers at
collectors and retailer. Of all (n=41) isolates of S. aureus were subjected against fourteen
antibiotics susceptibility testes by using the kirby-bauer disk diffusion method, high susceptible to
Ciprofloxacine (100%) Gentamycin(92.7), Erythromycin(92.7) and Kanamycine (90.2),while high
resistant S.aureus isolate to Penicillin G (100%) Ampicillin (85%) and Tetracycline (68.3) were
observed. The prevalence of multidrug resistance of S.aureus isolates from raw camel milk was
63.4% (26/41). Milk handling practice and consumer behavior at milk producers, sellers and
consumers would be assessed. A 60% of respondent had no clean milk container, 62.5 % could no
wash hand, 70% could no wash udders before milking and 57.5respondent were used plastic
containers under milk producers. Majority of sellers were pooling the milk obtain from different
producers and collectors that stored in plastic containers (86.7%) without refrigerator .A 100 % of
milk consumers had habit of raw milk consumption and have not awareness about milk borne
disease(100%) were observed at all respondents. The study showed that, high prevalence of S.aureus
from raw camel milk and multidrugs resistant were observed, which could be public health risk in
Babile district, East Hararge Zone. Creation of public awareness about good milk handling
practices, boiling of milk prior to consumption and periodic assessment of the drugs sensitivity test
prior to use.
