ISOLATION of SALMONELLA FROM LYMPHNODE, CARCASS, RECTUM and SKIN of CATTLE SLAUGHTERED at MUNICIPAL ABATTOIR; ANTIMICROBIAL SUSCEPTIBILITY PATTERN of SALMONELLA ISOLATES and FOOD SAFETY IMPLICATION: CHIRO TOWN EASTERN ETHIOPIA

Abstract

A wide range of pathogens play a role in food borne disease and most of them are zoonotic and have reservoirs in healthy food animals from which they spread to variety of foods. Among the pathogens, Salmonella is considered the most prevalent food borne pathogen worldwide and has long been recognized as an important zoonotic microorganism of economic and food safety implication in animals and humans. They cause acute gastroenteritis when ingested with contaminated food such as raw meat and meat products. They are also responsible for the emergence of antimicrobial resistance in both human and animals. So the aim of this study was to isolate, identify, to delineate the antimicrobial sensitivity pattern of non-typhoidal Salmonella and food safety implication. A cross-sectional study was conducted on slaughtered cattle carcass and slaughterhouse environment to isolate and conduct antimicrobial susceptibility pattern of salmonella. Systematic random samling technique were used to drawn sample from abattior. A total of 253 swab samples and tissue samples were systematically collected and examined for the presence of Salmonella following the standard techniques and procedures. The total number of samples from different sources including Sub-illiac lymph node (N = 253), out of which (n = 42) were Salmonella positive and this reveals that the total Salmonella prevalence in the study area was 16.6%. An antimicrobial susceptibility test was performed for all Salmonella positive isolates. Seven different antibiotic disks were used. From overall isolates, a high level of susceptibility was found for Gentamycin 42(100%) and Ciprofloxacin 42(100%). However, the highest resistances were found to Streptomycin 37(88.1%), Ampicillin 34(81.0%), and Amoxicillin 33(78.6%). The study showed that the occurrences of non-typhoidal Salmonella in the study area and antimicrobial resistance to the commonly used drugs. Therefore, adequate hygienic practices at abattoirs, applying food safety procedures and rational use of drug were recommended